Binding properties of antimicrobial agents to lipid membranes using surface plasmon resonance.

Lipid membrane-binding properties of daptomycin using surface plasmon resonance.

In the present study, we developed an assay of interactions of daptomycin with four model lipid membranes that mimicked mammal cell membranes and gram-positive and negative bacteria membranes. We also analyzed the binding kinetics of the gram-positive bacteria membranes using surface plasmon resonance (SPR). Daptomycin showed a higher affinity for the model gram-positive bacteria membrane than ...

Biol. Pharm. Bull. 28(1) 148—150 (2005)

naturally-occurring antimicrobial peptides provide a valuable tool for studying the role of different structural features in the activity of those peptides. A large array of derivatives based upon these structural models has been synthesized and studied in an attempt to increase the potency and selectivity of the native antimicrobial peptides. Enhancing the antimicrobial potency is often accomp...

Investigating the Effects of Some Capping Agents upon Gold Nanocrystals Stability, Size and Surface Plasmon Resonance

High-throughput and sensitive assay for amphotericin B interaction with lipid membrane on the model membrane systems by surface plasmon resonance.

In the present study, we developed a high-throughput and sensitive assay for interactions of amphotericin B (AmB) with two model lipid membranes, which mimicked mammal cell membrane and fungal membrane using surface plasmon resonance (SPR). The binding kinetics of AmB to the membrane could be analyzed by multiple sensorgrams obtained at different AmB concentrations, indicating that the binding ...

Structural and DNA-binding studies on the bovine antimicrobial peptide, indolicidin: evidence for multiple conformations involved in binding to membranes and DNA

Indolicidin, a l3-residue antimicrobial peptide-amide, which is unusually rich in tryptophan and proline, is isolated from the cytoplasmic granules of bovine neutrophils. In this study, the structures of indolicidin in 50% D3-trifluoroethanol and in the absence and presence of SDS and D38-dodecylphosphocholine were determined using NMR spectroscopy. Multiple conformations were found and were sh...